Journal: Current opinion in organ transplantation

Article Title: The dawn of liver perfusion machines

doi: 10.1097/MOT.0000000000000500

Figure Lengend Snippet: Ongoing normothermic machine perfusion clinical trials

Article Snippet: Normothermic Liver Preservation Trial , {"type":"clinical-trial","attrs":{"text":"NCT03089840","term_id":"NCT03089840"}} NCT03089840 , Alberta, Canada , Phase I/II; recruiting , 50 , SCD/ECD , OrganOx metra , Continuous or end-ischemic , Safety and feasibility , .

Techniques: Transplantation Assay, Preserving

Journal: Current opinion in organ transplantation

Article Title: The dawn of liver perfusion machines

doi: 10.1097/MOT.0000000000000500

Figure Lengend Snippet: Upcoming hypothermic machine perfusion with controlled oxygenated rewarming clinical trials

Article Snippet: Normothermic Liver Preservation Trial , {"type":"clinical-trial","attrs":{"text":"NCT03089840","term_id":"NCT03089840"}} NCT03089840 , Alberta, Canada , Phase I/II; recruiting , 50 , SCD/ECD , OrganOx metra , Continuous or end-ischemic , Safety and feasibility , .

Techniques:

Journal: bioRxiv

Article Title: Stearoyl-CoA Desaturases regulate stem and progenitor cell metabolism and function in response to nutrient abundance

doi: 10.1101/2025.09.17.676943

Figure Lengend Snippet: The requirement for SCD1 and SCD2 activity in intestinal stem cells was investigated by using mouse organoid models. (A) Established WT small intestinal organoids were treated with either vehicle (DMSO), or 250 nM of SCD inhibitors A939572 and CAY10566, or ACC1 inhibitor ND-646 for 96 hrs. (N=4). (B) The organoid forming capacity of stem cells and (C) the expression levels of intestinal stem cell markers were significantly decreased in inhibitor treated organoids which was investigated by RT-qPCR. (D) Organoids were collected and processed for EdU staining which fluorescently labeled proliferative cells. (E-F) Exogenous oleic acid addition (50 μM) rescued SCD inhibition (50 nM) phenotype, resulting in increased number of proliferative and differentiated organoids ex vivo (N=3-4). While SCD inhibition decreased the number of proliferative cells, addition of oleic acid exogenously rescued the Scd inhibition phenotype. (G) Small intestinal organoids were treated with either vehicle (DMSO) or SCD inhibitors A939572 or CAY10566 for four days. RT-qPCR was performed with organoids cDNA to investigate the expression levels of ER stress marker genes. (H) RT-qPCR was used to investigate the expression levels of Chop in small intestinal organoids under both oleic acid-treated and non-treated conditions, where Scd enzymes were inhibited. (I) WT small intestinal organoids were treated with either a DMSO vehicle, or 50 nM of the Scd inhibitor, A939572, for 24 hrs for lipidomics (N=3). Scale bar is 250μm. Graphs and images are representative of 3-4 mice, multiple wells and fields from each biological replicate are counted. Data are mean ± SD. *p<0.05, ***p<0.001, and ****p<0.0005.

Article Snippet: After desired size and budding morphology was maintained, organoids were harvested and incubated with 50 nM SCD inhibitor A939572 (Tocris) for 4 days.

Techniques: Activity Assay, Expressing, Quantitative RT-PCR, Staining, Labeling, Inhibition, Ex Vivo, Marker